Effects of Antrodia Camphorata Supplementation on Anti-fatigue Effects in Rats
補充樟芝對老鼠抗疲勞作用的影響
DOI:
https://doi.org/10.24112/ajper.161791Keywords:
Antrodia camphorate, exercise, 樟芝, 運動Abstract
LANGUAGE NOTE | Document text in English; abstract also in Chinese.
This study investigated the effects of antrodia camphorata (AC) supplementation on anti-fatigue effects after a single bout of exhaustive exercise in rats. Thirty-two rats were randomly divided into four groups: control (C, n=8); C with antrodia camphorata supplementation (A, n=8); exhaustive exercise (E, n=8); exhaustive exercise with AC supplementation (AE, n=8). Rats in groups A and AE received a 0.2 c.c. (kg/wt/day) AC supplementation for two weeks. Rats in groups E and AE performed an exhaustive running test on a treadmill at a final speed of 30m/min, 10% grade, at approximately 70-75% VO2max. Blood samples of each rat were collected. The concentrations of glucose, lactate dehydrogenase (LDH), uric acid, creatinine, and creatine kinase (CK) in blood were examined. The results showed a significant increase in exhaustive exercise time of AE group (78.79±9.09 min) compared with E group (67.77±9.22 min). The activities of CK, LDH, and blood creatinine concentration significantly increased in exercised rats (1227.40±553.24 U/I, 2108.32±513.99 U/I, 0.76±0.19 mg/ dl) compared with control group rats (358.98±125.53 U/I, 956.89±597.13 U/I, 0.5±0.15 mg/dl) and AE group rats (774.13±203.38 U/I, 1300.93±513.99 U/I, 0.5±0.05 mg/dl). The glucose in the plasma significantly decreased in E group rats (133.88±61.84 mg/dl) compared with C group rats (209.63±69.13 mg/dl) and AE group rats(188.88±60.32 mg/dl). The results indicate that AC supplementation could decrease CK, LDH activities. It may help for anti-fatigue effects.
本研究的目的在探討,樟芝補充劑對老鼠經單次衰竭運動後對抗疲勞作用的影響,三十二隻老鼠隨機分配為以下四組:控制組(C, n=8) 、樟芝組(A, n=8,補充樟芝,沒有運動)、運動組(E, n=8)、樟芝運動組(AE, n=8,補充樟芝,有運動),補充樟芝組(A & AE)的老鼠,每天補充每公斤體重0.2毫升的樟芝補充劑,持續兩週,衰竭運動組(E & AE)的老鼠需在鼠用跑步機上進行衰竭運動,運動強度約70~75%最大攝氧量,最終跑步機速度為30公尺/分鐘,坡度為10%。收集老鼠血液樣本,測量血糖、乳酸去氫酶、尿酸、肌酸酐、肌酸激酶。研究結果顯示AE組運動衰竭時間(78.79±9.09 min)顯著比E組(67.77±9.22 min)長。運動組(E)的肌酸激酶活性、乳酸去氫酶活性、肌酸酐(1227.40±553.24 U/I, 2108.32±513.99 U/I, 0.76±0.19 mg/dl)顯著比控制組(C)(358.98± 125.53 U/I, 956.89±597.13 U/I, 0.5±0.15 mg/dl)及樟芝運動組(AE)高(774.13±203.38 U/I, 1300.93±513.99 U/ I, 0.5±0.05 mg/dl)。運動組(E)血糖濃度(133.88±61.84 mg/dl)顯著比控制組(209.63±69.13 mg/dl)及樟芝運動組(188.88±60.32 mg/dl)低。研究結果表示樟芝補充能夠降低肌酸激酶活性及乳酸去氫酶活性,有助於抗疲勞的效用。
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